Pu-miR172d regulates stomatal density and water use efficiency via targeting PuGTL1 in poplar.
Identifieur interne : 000167 ( Main/Exploration ); précédent : 000166; suivant : 000168Pu-miR172d regulates stomatal density and water use efficiency via targeting PuGTL1 in poplar.
Auteurs : Quangang Liu [République populaire de Chine] ; Zhanchao Wang [République populaire de Chine] ; Sen Yu [République populaire de Chine] ; Wenlong Li [République populaire de Chine] ; Mengqiu Zhang [République populaire de Chine] ; Jia Yang [République populaire de Chine] ; Dandan Li [République populaire de Chine] ; Jingli Yang [République populaire de Chine] ; Chenghao Li [République populaire de Chine]Source :
- Journal of experimental botany [ 1460-2431 ] ; 2020.
Abstract
miRNAs play essential regulatory roles in many aspects of plant development and responses to abiotic and biotic environments. Here, we characterized Pu-miR172d, which acts as a negative regulator of stomatal density by directly repressing the expression of PuGTL1 in Populus ussuriensis. quantitative real-time PCR (RT-qPCR) and GUS reporter analyses showed that Pu-miR172d was strongly expressed in the guard cells of young leaves. Pu-miR172d overexpression significantly decreased stomatal density, resulting in the increase of WUE and drought tolerance by reducing net photosynthetic rate, stomatal conductance, and transpiration. Molecular analysis showed that PuGTL1 was a major target of Pu-miR172d cleavage. Moreover, PuGTL1-SRDX plants, in which PuGTL1 is suppressed, phenocopied Pu-miR172d overexpression lines with reduced stomatal density and enhanced WUE. In addition, the expression level of PuSDD1, a negative regulator of stomatal development, was significantly increased in young leaves of both Pu-miR172d overexpression and PuGTL1-SRDX plants. RNA-seq analysis of mature leaves revealed that Pu-miR172d overexpression decreased the expression of many genes related to photosynthesis. Our findings show that the Pu-miR172d/PuGTL1/PuSDD1 module plays an important role in stomatal differentiation, indicating its ability to improve the engineering of drought tolerance in poplar.
DOI: 10.1093/jxb/eraa493
PubMed: 33098429
Affiliations:
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type="wicri:Area/Main/Exploration">000041</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Pu-miR172d regulates stomatal density and water use efficiency via targeting PuGTL1 in poplar.</title><author><name sortKey="Liu, Quangang" sort="Liu, Quangang" uniqKey="Liu Q" first="Quangang" last="Liu">Quangang Liu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Shenyang Agricultural University, Shenyang, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Shenyang Agricultural University, Shenyang</wicri:regionArea><wicri:noRegion>Shenyang</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Zhanchao" sort="Wang, Zhanchao" uniqKey="Wang Z" first="Zhanchao" last="Wang">Zhanchao Wang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Sen" sort="Yu, Sen" uniqKey="Yu S" first="Sen" last="Yu">Sen Yu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Li, Wenlong" sort="Li, Wenlong" uniqKey="Li W" first="Wenlong" last="Li">Wenlong Li</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Mengqiu" sort="Zhang, Mengqiu" uniqKey="Zhang M" first="Mengqiu" last="Zhang">Mengqiu Zhang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Jia" sort="Yang, Jia" uniqKey="Yang J" first="Jia" last="Yang">Jia Yang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Li, Dandan" sort="Li, Dandan" uniqKey="Li D" first="Dandan" last="Li">Dandan Li</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Jingli" sort="Yang, Jingli" uniqKey="Yang J" first="Jingli" last="Yang">Jingli Yang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Li, Chenghao" sort="Li, Chenghao" uniqKey="Li C" first="Chenghao" last="Li">Chenghao Li</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of experimental botany</title><idno type="eISSN">1460-2431</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">miRNAs play essential regulatory roles in many aspects of plant development and responses to abiotic and biotic environments. Here, we characterized Pu-miR172d, which acts as a negative regulator of stomatal density by directly repressing the expression of PuGTL1 in Populus ussuriensis. quantitative real-time PCR (RT-qPCR) and GUS reporter analyses showed that Pu-miR172d was strongly expressed in the guard cells of young leaves. Pu-miR172d overexpression significantly decreased stomatal density, resulting in the increase of WUE and drought tolerance by reducing net photosynthetic rate, stomatal conductance, and transpiration. Molecular analysis showed that PuGTL1 was a major target of Pu-miR172d cleavage. Moreover, PuGTL1-SRDX plants, in which PuGTL1 is suppressed, phenocopied Pu-miR172d overexpression lines with reduced stomatal density and enhanced WUE. In addition, the expression level of PuSDD1, a negative regulator of stomatal development, was significantly increased in young leaves of both Pu-miR172d overexpression and PuGTL1-SRDX plants. RNA-seq analysis of mature leaves revealed that Pu-miR172d overexpression decreased the expression of many genes related to photosynthesis. Our findings show that the Pu-miR172d/PuGTL1/PuSDD1 module plays an important role in stomatal differentiation, indicating its ability to improve the engineering of drought tolerance in poplar.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33098429</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>24</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1460-2431</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Oct</Month><Day>24</Day></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J Exp Bot</ISOAbbreviation></Journal><ArticleTitle>Pu-miR172d regulates stomatal density and water use efficiency via targeting PuGTL1 in poplar.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">eraa493</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1093/jxb/eraa493</ELocationID><Abstract><AbstractText>miRNAs play essential regulatory roles in many aspects of plant development and responses to abiotic and biotic environments. Here, we characterized Pu-miR172d, which acts as a negative regulator of stomatal density by directly repressing the expression of PuGTL1 in Populus ussuriensis. quantitative real-time PCR (RT-qPCR) and GUS reporter analyses showed that Pu-miR172d was strongly expressed in the guard cells of young leaves. Pu-miR172d overexpression significantly decreased stomatal density, resulting in the increase of WUE and drought tolerance by reducing net photosynthetic rate, stomatal conductance, and transpiration. Molecular analysis showed that PuGTL1 was a major target of Pu-miR172d cleavage. Moreover, PuGTL1-SRDX plants, in which PuGTL1 is suppressed, phenocopied Pu-miR172d overexpression lines with reduced stomatal density and enhanced WUE. In addition, the expression level of PuSDD1, a negative regulator of stomatal development, was significantly increased in young leaves of both Pu-miR172d overexpression and PuGTL1-SRDX plants. RNA-seq analysis of mature leaves revealed that Pu-miR172d overexpression decreased the expression of many genes related to photosynthesis. Our findings show that the Pu-miR172d/PuGTL1/PuSDD1 module plays an important role in stomatal differentiation, indicating its ability to improve the engineering of drought tolerance in poplar.</AbstractText><CopyrightInformation>© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Quangang</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Forestry, Shenyang Agricultural University, Shenyang, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Zhanchao</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Sen</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Wenlong</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Mengqiu</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Jia</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Dandan</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Jingli</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Chenghao</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Populus ussuriensis
</Keyword><Keyword MajorTopicYN="N">GT2-LIKE 1</Keyword><Keyword MajorTopicYN="N">drought tolerance</Keyword><Keyword MajorTopicYN="N">microRNA172d</Keyword><Keyword MajorTopicYN="N">stomatal density</Keyword><Keyword MajorTopicYN="N">water use efficiency</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>07</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>10</Month><Day>24</Day><Hour>12</Hour><Minute>6</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>10</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33098429</ArticleId><ArticleId IdType="pii">5937353</ArticleId><ArticleId IdType="doi">10.1093/jxb/eraa493</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Liu, Quangang" sort="Liu, Quangang" uniqKey="Liu Q" first="Quangang" last="Liu">Quangang Liu</name></noRegion><name sortKey="Li, Chenghao" sort="Li, Chenghao" uniqKey="Li C" first="Chenghao" last="Li">Chenghao Li</name><name sortKey="Li, Dandan" sort="Li, Dandan" uniqKey="Li D" first="Dandan" last="Li">Dandan Li</name><name sortKey="Li, Wenlong" sort="Li, Wenlong" uniqKey="Li W" first="Wenlong" last="Li">Wenlong Li</name><name sortKey="Liu, Quangang" sort="Liu, Quangang" uniqKey="Liu Q" first="Quangang" last="Liu">Quangang Liu</name><name sortKey="Wang, Zhanchao" sort="Wang, Zhanchao" uniqKey="Wang Z" first="Zhanchao" last="Wang">Zhanchao Wang</name><name sortKey="Yang, Jia" sort="Yang, Jia" uniqKey="Yang J" first="Jia" last="Yang">Jia Yang</name><name sortKey="Yang, Jingli" sort="Yang, Jingli" uniqKey="Yang J" first="Jingli" last="Yang">Jingli Yang</name><name sortKey="Yu, Sen" sort="Yu, Sen" uniqKey="Yu S" first="Sen" last="Yu">Sen Yu</name><name sortKey="Zhang, Mengqiu" sort="Zhang, Mengqiu" uniqKey="Zhang M" first="Mengqiu" last="Zhang">Mengqiu Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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